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网站首页 >> pET-35b大肠表达质粒 货号：P1100
pET-35b大肠表达质粒 货号：P1100

信息

别名 pET35b
启动子 T7
复制子 pBR322
终止子 T7 terminator
质粒分类 大肠杆菌载体；PET系列表达质粒
质粒大小 5927bp
质粒标签 N-CBDclos,C-6×His
原核抗性 Kan
克隆菌株 DH5α
培养条件 37℃
表达宿主 BL21(DE3)
培养条件 37℃，有氧，LB
诱导方式 IPTG或乳糖及其类似物
5'测序引物 T7:TAATACGACTCACTATAGGG
3'测序引物 T7-ter:TGCTAGTTATTGCTCAGCGG
质粒宿主 大肠杆菌
质粒用途 蛋白表达
片段类型 基因CDS
片段物种 空载体
原核抗性 氨苄青霉素

简介
       The pET-35b(+) vector is designed for expression of CBDclos fusion proteins.A variety of cloning sites and strategies are available. Rapid directional cloning of PCR-amplified DNA is available with the pET-35 Xa/LIC Vector Kit, which contains linearized pET-35b(+) ready for annealing with appropriately prepared inserts. When cloned with the Xa/LIC method, resulting CBDclos•Tag™ fusion proteins can be cleaved precisely at the vector-encoded junction using Factor Xa. Unique sites are shown on the circle map. Note that the sequence is num-bered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer.


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garmin 230
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