DH5αλpir大肠杆菌克隆菌株 货号:st019 DH5αλpir大肠杆菌。E. coli DH5α λpir strain contains the pir gene (lysogenized lpir phage lysogen) and is designed for cloning and propagation of plasmids with R6K origin of replication sequence (like pUTmini-Tn5 vectors). To transfer this plasmids directly from DH5α λpir donor strain to the recipient strain it is necessary to perform a triparental mating with a helper strain such as E. coli DH5α (pRK2013) (see Helper E. coli strains). The genotype of the strain is sup E44, ΔlacU169 (ΦlacZΔM15), recA1, endA1, hsdR17, thi-1, gyrA96, relA1, λpir phage lysogen.