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网站首页 >> pBAD18大肠表达质粒 货号：P1185
pBAD18大肠表达质粒 货号：P1185

信息

别名 pBAD-18
启动子 araBAD
复制子 pBR322
质粒分类 大肠杆菌载体；pBAD系列表达质粒
质粒大小 4613bp
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 推荐LMG194
培养条件 37℃，有氧，LB
诱导方式 阿拉伯糖
5'测序引物 pBAD-F: ATGCCATAGCATTTTTATCC
3'测序引物 pBAD-R: GATTTAATCTGTATCAGG
质粒宿主 大肠杆菌
质粒用途 蛋白表达
片段类型 基因CDS
片段物种 空载体
原核抗性 氨苄青霉素

简介
         This vector is one of several tightly controlled expression vectors regulated by the arabinose operon. Vectors differ in replicon, antibiotic resistance marker, multiple cloning site and need for translation initiation sequences. Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerolcontaining media by the addition of arabinose. Expression is repressed by addition of glucose or other catabolites. Cloned inserts must provide a translation initiation sequence (ATG) and ribosome binding site for expression. Expression of cloned inserts in the two recommended hosts differ in terms of induction characteristics, especially when using low concentrations of inducer (arabinose) and growing in minimal media. The following primers can be used for sequencing of cloned inserts: 5 primer (278bp upstream of the NheI site) :5 CTGTTTCTCCATACCCGTT 3and one of two 3 primers:3 primer 1 (219bp downstream of the HindIII site):5 CTCATCCGCCAAAACAG 33 primer 2 (1733bp downstream of the HindIII site):5 GGCTGAAAATCTTCTCT3

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garmin 230
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