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网站首页 >> pTWIN2质粒 货号:P1290
pTWIN2质粒 货号:P1290

信息

启动子: T7
复制子: M13 ori
质粒分类: 大肠杆菌载体；蛋白表达质粒
质粒大小: 7192bp
原核抗性: 氨苄青霉素Amp
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: 大肠杆菌
培养条件: 37℃，有氧，LB
诱导方式: IPTG或乳糖及其类似物
5'测序引物: T7:TAATACGACTCACTATAGGG
3'测序引物: 根据序列设计
备注: 表达质粒

简介

pTWIN2 is an E. coli expression vector which can be used with the IMPACT? Kit. A polylinker in the vector is designed for the in-frame fusion of a target gene between the modified Ssp DnaB (2) and Mth RIR1 inteins (3). The presence of the chitin binding domain from Bacillus circulans (4,5) facilitates purification. pTWIN vectors are designed for protein purification or for the isolation of proteins with an N-terminal cysteine and/or a C-terminal thioester (1). The double-stranded vector is 7192 base pairs in length

图谱

garmin 230