pCDNA3.0载体是专门设计用来在哺乳细胞中进行高水平的稳定表达和瞬时表达。其中人巨细胞病毒早期启动子(CMV)在广泛的哺乳动物细胞中可以实现高表达。另外为筛选稳定细胞系提供了新霉素抗性基因,可用G418筛选。
pcDNA3.0 is 5.4 kb vectors designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells. Neomycin resistance gene for selection of stable cell lines.
pcDNA3.0 vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin selectable marker and a forward-orientation multiple cloning site. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells Multiple cloning sites orientations to facilitate cloning .Neomycin resistance gene for selection of stable cell lines Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen.
特征片段:
CMV promoter: bases 209-863 T7 promoter: bases 864-882
Polylinker: bases 889-994 Sp6 promoter: bases 999-1016
BGH poly A: bases 1018-1249 SV40 promoter: bases 1790-2115
SV40 origin of replication: bases 1984-2069 Neomycin ORF: bases 2151-2945
SV40 poly A: bases 3000-3372 ColE1 origin: bases 3632-4305
Ampicillin ORF: bases 4450-5310