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pCDNA3.0哺乳表达质粒 货号:P2001

信息

别名 pCDNA3
启动子 CMV
复制子 pUC
终止子 bGH poly(A) signal
质粒分类 哺乳系列质粒;哺乳表达质粒;pCDNA系列质粒
质粒大小 5446bp
质粒标签
原核抗性 Amp
真核抗性 G418
克隆菌株 DH5α
培养条件 37℃
表达宿主 293T等哺乳细胞
培养条件 37℃,5%CO2
诱导方式 无需诱导
5'测序引物 pCDNA3.1-F(CTAGAGAACCCACTGCTTAC)
3'测序引物 pCDNA3.1-R(TAGAAGGCACAGTCGAGG)
备注 常用的哺乳细胞真核表达载体
质粒宿主 哺乳细胞
质粒用途 蛋白表达
片段类型 基因CDS
片段物种 空载体
原核抗性 氨苄青霉素


简介
        pCDNA3.0载体是专门设计用来在哺乳细胞中进行高水平的稳定表达和瞬时表达。其中人巨细胞病毒早期启动子(CMV)在广泛的哺乳动物细胞中可以实现高表达。另外为筛选稳定细胞系提供了新霉素抗性基因,可用G418筛选。
        pcDNA3.0 is 5.4 kb vectors  designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.  Neomycin resistance gene for selection of stable cell lines.
        pcDNA3.0 vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin selectable marker and a forward-orientation multiple cloning site. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells Multiple cloning sites orientations to facilitate cloning .Neomycin resistance gene for selection of stable cell lines Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen.
特征片段:
                        CMV promoter: bases 209-863                                    T7 promoter: bases 864-882
                        Polylinker: bases 889-994                                            Sp6 promoter: bases 999-1016
                        BGH poly A: bases 1018-1249                                     SV40 promoter: bases 1790-2115
                        SV40 origin of replication: bases 1984-2069                Neomycin ORF: bases 2151-2945
                        SV40 poly A: bases 3000-3372                                    ColE1 origin: bases 3632-4305
                        Ampicillin ORF: bases 4450-5310



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