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网站首页 >> pCDNA3.1-Myc-HisB(-)哺乳表达质粒 货号：P2015
pCDNA3.1-Myc-HisB(-)哺乳表达质粒 货号：P2015

信息

别名 pCDNA3.1(-)-Myc-HisB
启动子 CMV
复制子 pUC
终止子 BGH poly（A）signal
质粒分类 哺乳细胞载体;蛋白过表达质粒
质粒大小 5520bp
质粒标签 6X His, myc
原核抗性 Amp
真核抗性 G418
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
质粒宿主 哺乳细胞
质粒用途 蛋白表达
片段类型 基因CDS
片段物种 空载体
原核抗性 氨苄青霉素

简介
pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.  


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