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pCAGGS-HA哺乳表达质粒 货号:P2059

信息

别名 pCAGGS-HA-N
启动子 CAG
复制子 pUC
质粒分类 哺乳系列质粒;哺乳表达质粒;pCAG系列质粒
质粒大小 4807bp
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 293T等哺乳细胞
培养条件 37℃,5%CO2
诱导方式 无需诱导
5'测序引物 pcaggs-F(GCTAACCATGTTCATGCCTTCT)
3'测序引物 pcaggs-R(TATGTCCTTCCGAGTGAGAG)
质粒宿主 哺乳细胞
质粒用途 蛋白表达
片段类型 基因CDS
片段物种 空载体
原核抗性 氨苄青霉素


简介
          pCAGGS-HA质粒是在pCAGGS载体的基础上添加了HA标签,利于在WB实验的时候用抗体检测。This plasmid is useful for highly efficient expression of genes under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.The AG promoter sequence consists of the chicken β-actin promoter, the first exon and part of the first intron (that seems to have a strong enhancer-like activity) linked to a rabbit β-globin fragment, consisting of a 3' part of the second intron (inclusive a branch point which is required for normal splicing reactions) and a 5' part of the third exon.When cloning a fragment downstream from the lac promoter it may be advisable to use lacIq strains in order to prevent fortuitous expression of a possibly noxious polypeptide.


图谱



garmin 230