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网站首页 >> pmCherry-C1哺乳荧光质粒 货号：P2098
pmCherry-C1哺乳荧光质粒 货号：P2098

信息

启动子 CMV
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞，荧光蛋白报告载体
质粒大小 4722bp
原核抗性 Kan
真核抗性 G418
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 mCherry-F:AACGAGGACTACACCATCGT
3'测序引物 Sv40-polyA-R：GAAATTTGTGATGCTATTGC
质粒宿主 哺乳细胞
质粒用途 蛋白表达
片段类型 基因CDS
原核抗性 卡那霉素

简介
      pmCherry-C1 is a mammalian expression vector designed to express a protein of interest fused to the C-terminus of mCherry, a mutant fuorescent protein derived from the tetrameric Discosoma sp. red fuorescent protein, DsRed (1). The excitation and emission maxima of the native mCherry protein are 587 nm and 610 nm, respectively. Expression of fusion proteins that retain the fuorescent properties of the unmodifed mCherry protein can be monitored by fow cytometry and their localization in vivo can be determined by fuorescence microscopy.


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garmin 230