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网站首页 >> pEYFP-Golgi高尔基体定位质粒 货号：P2114
pEYFP-Golgi高尔基体定位质粒 货号：P2114

信息

启动子 CMV
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞，荧光蛋白报告载体
质粒大小 4918bp
原核抗性 Kan
真核抗性 G418
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物 根据序列设计引物
质粒宿主 哺乳细胞
质粒用途 定位
原核抗性 卡那霉素

简介
     pEYFP-Golgi encodes a fusion protein consisting of enhanced yellow fluorescent protein (EYFP) and a sequence encoding the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase (GT). This region of human beta 1,4-GT contains the membrane-anchoring signal peptide that targets the fusion protein to the trans-medial region of the Golgi apparatus. The EYFP gene contains the four amino acid substitutions previously published as GFP-10C. The fluorescence excitation maximum of EYFP is 513 nm; the emission spectrum has a peak at 527 nm (in the yellow-green region). When excited at 514 nm, the Em of EYFP is 84,500 cm-1M-1 and the fluorescence quantum yield is 0.61, resulting in a bright fluorescent signal. The fluorescence observed is roughly equivalent to that of EGFP.


图谱



garmin 230