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网站首页 >> pECFP-C1哺乳荧光质粒 货号：P2121
pECFP-C1哺乳荧光质粒 货号：P2121

信息

启动子 CMV
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞，荧光蛋白报告载体
质粒大小 4731bp
原核抗性 Kan
真核抗性 G418
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物 Sv40-polyA-R：GAAATTTGTGATGCTATTGC
质粒宿主 哺乳细胞
质粒用途 蛋白表达
片段类型 基因CDS
原核抗性 卡那霉素

简介
     pECFP-C1 encodes an enhanced cyan fluorescent variant of the Aequorea victoria green fluorescent protein gene (GFP). The ECFP gene contains six amino acid substitutions. The Tyr-66 to Trp substitution gives ECFP fluorescence excitation (major peak at 433 nm and a minor peak at 453 nm) and emission (major peak at 475 nm and a minor peak at 501 nm) similar to other cyan emission variants (1–3). The other five substitutions (Phe-64 to Leu; Ser-65 to Thr; Asn-146 to Ile; Met-153 to Thr; and Val-163 to Ala) enhance the brightness and solubility of the protein, primarily due to improved protein-folding properties and efficiency of chromophore formation (2, 4, 5).


图谱



garmin 230