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网站首页 >> pGL4.29[luc2P-CRE-Hygro]哺乳报告质粒 货号：P2141
pGL4.29[luc2P-CRE-Hygro]哺乳报告质粒 货号：P2141

信息

启动子 SV40
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞，信号通路报告载体
质粒大小 6084bp
原核抗性 Amp
真核抗性 Hyg
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 RVP3:CTAGCAAAATAGGCTGTCCC
3'测序引物 根据序列设计引物
质粒宿主 哺乳细胞
质粒用途 信号报告
片段类型 启动子
片段物种 空载体
原核抗性 氨苄青霉素
真核抗性 潮霉素
荧光标记 Fluc

简介
       The pGL4.29[luc2P/CRE/Hygro] Vector(a–f) contains a cAMP response element (CRE) that drives the transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance


图谱



garmin 230