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网站首页 >> pRL-TK哺乳报告质粒 货号：P2148
pRL-TK哺乳报告质粒 货号：P2148

信息

启动子 TK
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞，信号通路报告载体
质粒大小 4045bp
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 T7:TAATACGACTCACTATAGGG
3'测序引物 根据序列设计引物
质粒宿主 哺乳细胞
质粒用途 信号报告
原核抗性 氨苄青霉素
荧光标记 Fluc

简介
    The pRL-TK Vector may be used in combination with any experimental reporter vector to cotransfect mammalian cells. However, it is important to realize that trans effects between promoters on cotransfected plasmids can potentially affect reporter gene expression. This is primarily of concern when either the control or experimental reporter vector, or both, contain very strong promoter/enhancer elements.The occurrence and magnitude of such effects will depend on several factors: i) the combination and activities of the genetic regulatory elements present on the cotransfected vectors, ii) the relative ratio of experimental vector to control vector introduced into the cells, and iii) the type of cell transfected.


图谱



garmin 230