pACT-MyoD哺乳双杂交质粒 货号：P2199
来源:
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作者:shhebio
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发布时间: 2017-12-08
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359 次浏览
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分享到:
信息
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启动子
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CMV
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复制子
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pUC
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终止子
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SV40 poly(A) signal
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质粒分类
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哺乳细胞，哺乳细胞双杂交载体
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质粒大小
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7400bp
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原核抗性
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Amp
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克隆菌株
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DH5α
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培养条件
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37℃
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表达宿主
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哺乳细胞
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诱导方式
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无须诱导，瞬时表达
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5'测序引物
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CMV-F：CGCAAATGGGCGGTAGGCGTG
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3'测序引物
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根据序列设计引物
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质粒宿主
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哺乳细胞
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质粒用途
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杂交
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原核抗性
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氨苄青霉素
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简介
The CheckMate™ Mammalian Two-Hybrid System provides positive control vectors that encode and express two proteins known to interact in vivo (13). MyoD is expressed in skeletal muscle and is a myogenic regulatory protein (14,15). The Id protein acts as a negative regulator of myogenic differentiation (16). MyoD and Id are members of the helix-loop-helix family of nuclear proteins. The pBIND-Id Control Vector contains a segment of murine Id cDNA (amino acids 29–148; 13,16) cloned in-frame with the GAL4 DNA-binding domain of the pBIND Vector . The pACT-MyoD Control Vector contains a segment of murine MyoD cDNA (amino acids 1–318; 13,15) cloned in-frame with the VP16 activation domain of the pACT Vector .
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