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网站首页 >> Super PiggyBac Transposase (PB200PA-1) 哺乳转座质粒 货号：P2215
Super PiggyBac Transposase (PB200PA-1) 哺乳转座质粒 货号：P2215

信息

启动子 Polr2A promoter
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞转座酶表达质粒
质粒大小 8531bp
原核抗性 Amp
克隆菌株 Stbl3
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 M13R:CAGGAAACAGCTATGACC
3'测序引物 根据序列设计引物
质粒宿主 哺乳细胞
质粒用途 转座
原核抗性 氨苄青霉素

简介
     The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors andchromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposonspecific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the piggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes.The Super PiggyBac transposase expression vector features a 5’ HS4 insulator to support robust transcription from the rPolr2A promoter. The PiggyBac transposase coding sequence has been optimized for high expression, stability and activity in mammalian cells.

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