咨询热线：​​​
17317861055
1
1

17317861055

24小时销售服务热线：

1

我们的产品

Product

+

1

您现在的位置：

网站首页 >> pSecTag2A哺乳分泌质粒 货号：P2340
pSecTag2A哺乳分泌质粒 货号：P2340

信息

启动子 CMV
复制子 pUCFI
终止子 SV40 poly(A) signal,BGH poly(A) signal
质粒大小 5159bp
原核抗性 Amp
真核抗性 Zeo
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 T7:TAATACGACTCACTATAGGG
3'测序引物 BGH:TAGAAGGCACAGTCGAGG
质粒宿主 哺乳细胞
质粒用途 蛋白表达
原核抗性 氨苄青霉素
真核抗性 博来霉素

简介
      pSecTag2 A, B, and C vectors are fusion vectors requiring that you clone your gene of interest in frame with the initiation ATG of the N-terminal Igκ-chain leader sequence and/or the C-terminal myc epitope/polyhistidine tag. Three versions of this vector are provided to facilitate cloning. For proper expression,first determine which restriction sites are appropriate for ligation and then which vector will preserve the reading frame at BOTH the 5′ and the 3′ ends. It may be nece ssary to PCR your gene product to create a fragment with the appropriate restriction sites to clone in frame at both ends. Carefully inspect your gene and the multiple cloning site of each vector before cloning your gene of interest


图谱


garmin 230