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网站首页 >> pTet-On-Advanced哺乳调控质粒 货号：P2343
pTet-On-Advanced哺乳调控质粒 货号：P2343

信息

启动子 CMV
复制子 pUC
终止子 SV40 poly(A) signal，beta-globin polyA signal
质粒分类 哺乳细胞，四环素调控系统载体
质粒大小 7139bp
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 四环素诱导
5'测序引物 CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物 根据序列设计引物
质粒宿主 哺乳细胞
质粒用途 蛋白表达
原核抗性 氨苄青霉素

简介
     pTet-On-Advanced expresses an improved version of the reverse Tet (tetracycline)-controlled transactivator protein (rtTA), called rtTA-Advanced (1–4). It is more sensitive to doxycycline (Dox) and yields lower background expression than the original rtTA used in the Tet-On® System (2). The rtTA-Advanced protein is a fusion of amino acids 1–207 of a mutant Tet repressor (TetR) and 39 amino acids containing three minimal "F"-type transcriptional activation domains from the VP16 protein of herpes simplex virus. It is fully synthetic, lacks cryptic splice sites, and is codon-optimized for stable expression in mammalian cells.


 

图谱



garmin 230