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网站首页 >> pTet-On哺乳调控质粒 货号：P2344
pTet-On哺乳调控质粒 货号：P2344

信息

启动子 CMV
复制子 pUC
终止子 SV40 poly(A) signal，beta-globin polyA signal
质粒分类 哺乳细胞，四环素调控系统载体
质粒大小 7400bp
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 四环素诱导
5'测序引物 CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物 根据序列设计引物
质粒宿主 哺乳细胞
质粒用途 蛋白表达
原核抗性 氨苄青霉素

简介
     pTet-On® expresses the reverse tet-responsive transcriptional activator (rtTA) from the strong immediate early promoter of cytomegalovirus (PCMV). tTA is a fusion of amino acids 1–207 of the tet repressor (TetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of herpes simplex virus. pTet-On is similar to pTet-Off except for four amino acid changes that convert the TetR to a rTetR, and consequently, tTA to rtTA. (pTet-Off also contains several silent mutations.) pTet-On was originally described as pUHD17-1neo by Gossen et al. (1995). pTet-On can be distinguished from pTet-Off by digestion with Hind III.


图谱




garmin 230