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网站首页 >> pRevTRE哺乳调控质粒 货号：P2346
pRevTRE哺乳调控质粒 货号：P2346

信息

启动子 CMV
复制子 pUC
质粒分类 哺乳细胞，四环素调控系统载体
质粒大小 6487bp
原核抗性 Amp
真核抗性 Hyg
克隆菌株 Stbl3
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 四环素诱导
5'测序引物 根据序列设计引物
3'测序引物 根据序列设计引物
质粒宿主 哺乳细胞
质粒用途 蛋白表达
原核抗性 氨苄青霉素
真核抗性 潮霉素

简介
      pRevTRE is a retroviral Tet response vector that expresses a gene of interest from the Tetresponse element (TRE). This vector is derived from pLNCX, a retroviral vector created using elements of Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV) as described (1). The TRE contains seven direct repeats of the tetO operator sequence, upstream of a minimal CMV promoter, which can be bound by the tTA and rtTA transactivators. The 5' viral LTR controls expression of the transcript that contains Ψ+ (the extended viral packaging signal) and the hygromycin resistance (Hygr) gene for antibiotic selection in mammalian cells. The TRE is derived from vectors described previously (2, 3). pRevTRE also includes the E. coli Ampr gene for antibiotic selection in bacteria.

图谱

garmin 230