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网站首页 >> pCMV-Tet3G哺乳调控质粒 货号：P2348
pCMV-Tet3G哺乳调控质粒 货号：P2348

信息

启动子 CMV
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞，四环素调控系统载体
质粒大小 7139bp
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 四环素诱导
5'测序引物 CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物 根据序列设计引物
质粒宿主 哺乳细胞
质粒用途 蛋白表达
原核抗性 氨苄青霉素

简介
     The pCMV-Tet3G Vector expresses Tet-On® 3G, a tetracycline-controlled transactivator that exhibits high activity in the presence of the inducer doxycycline (Dox) and exceptionally low activity in its absence. Tet-On 3G results from the fusion of amino acids 1–207 of a mutant Tet repressor (TetR) to 39 amino acids that form three minimal "F"-type transcriptional activation domains from the herpes simplex virus VP16 protein. Tet-On 3G was derived from Tet-On Advanced (1–4); as a result, it’s fully synthetic, lacks cryptic splice sites, and is codon-optimized for stable expression in mammalian cells. Compared to both of its predecessors, this 3rd generation Tet-On transactivator demonstrates increased sensitivity to Dox (1). Constitutive expression of Tet-On 3G is driven by the human cytomegalovirus immediately early promoter (PCMV IE). Note: An EF1α version of this vector is available for cell lines in which the CMV promoter is silenced.
     pCMV-Tet3G is used to develop stable Tet-On 3G cell lines, which are hosts for Tet-inducible gene expression systems. To create a Tet-inducible expression system, a vector containing a gene of interest under the control of the Tet-inducible TRE3G promoter (PTRE3G) is transfected into a Tet-On 3G cell line. The addition of Dox to the system causes Tet-On 3G to undergo a conformational change that allows it to bind to PTRE3G, activating transcription of the gene of interest in a highly dose-dependent manner


图谱



garmin 230