The pGenesil-1 plasmid was obtained by the transformation of pEGFP-C1 plasmid. PEGFP-C1 plasmid has been widely used in mammalian cells for many years, it has fluorescent protein expression, it is very convenient to detect the effect of. When your target gene was not detected by conventional fluorescence detection, the pGenesil-1 plasmid encoding shRNAs was a better choice.