pRS4xx系列质粒被用于基因的穿梭载体克隆酿酒酵母,但能够在大肠杆菌中复制。虽然在大肠杆菌中,他们从pMB1的复制原点复制至pBR322。他们携带的bla(APR)标记用于选择用氨苄青霉素和复制的噬菌体F1原点单链DNA 。pRS403
through pRS406 are integrative plasmids (YIp); pRS413through pRS416 (shown
below) are centromeric plasmids (YCp),and differ from the YIp by the insertion
of a centromere (CEN)and autonomously replicating sequence (ARS); pRS423
throughpRS426 are episomal plasmids (YEp), and carry instead theorigin of
replication from the yeast 2µ circle plasmid along withREP3 and FRT sequences
(2). The 4 vectors in each series differonly in the yeast auxotrophic marker
used: HIS3 (pRS4x3), TRP1(pRS4x4), LEU2 (pRS4x5) or URA3 (pRS4x6).