咨询热线:
17317861055
1
1

17317861055

24小时销售服务热线:

1

我们的产品

Product

+

1

您现在的位置:

pRS403酿酒酵母质粒 货号:P5040

信息

复制子 pUCFI
质粒分类 酵母系列质粒;酵母表达质粒;酿酒酵母质粒
质粒大小 4456bp
原核抗性 Amp
真核抗性 HIS3
克隆菌株 DH5α
培养条件 37℃
表达宿主 YPH499,YPH500等酿酒酵母
培养条件 30℃,YPD,有氧
5'测序引物 T7(TAATACGACTCACTATAGGG)
3'测序引物 M13R(CAGGAAACAGCTATGACC)
质粒宿主 酵母菌
质粒用途 蛋白表达
原核抗性 氨苄青霉素
真核抗性 HIS3


简介

    pRS4xx系列质粒被用于基因的穿梭载体克隆酿酒酵母,但能够在大肠杆菌中复制。虽然在大肠杆菌中,他们从pMB1的复制原点复制至pBR322。他们携带的blaAPR)标记用于选择用氨苄青霉素和复制的噬菌体F1原点单链DNA pRS403 through pRS406 are integrative plasmids (YIp); pRS413through pRS416 (shown below) are centromeric plasmids (YCp),and differ from the YIp by the insertion of a centromere (CEN)and autonomously replicating sequence (ARS); pRS423 throughpRS426 are episomal plasmids (YEp), and carry instead theorigin of replication from the yeast 2µ circle plasmid along withREP3 and FRT sequences (2). The 4 vectors in each series differonly in the yeast auxotrophic marker used: HIS3 (pRS4x3), TRP1(pRS4x4), LEU2 (pRS4x5) or URA3 (pRS4x6).



图谱



garmin 230