蜡状芽孢杆菌的启动子捕获质粒pAD123,该质粒无启动子,其编码绿色荧光蛋白GFPmut3a的突变型,其针对荧光激活细胞分选进行优化Cormack,绿色荧光蛋白(GFP)的FACS优化突变体)。
We constructed a
promoter-trap plasmid, pAD123, for Bacillus cereus. This plasmid contains a
promoterless gene that encodesa mutant version of the green fluorescent
protein, GFPmut3a, that is optimized for fluorescence-activated cell sorting
[Cormack, FACS-optimized mutants of the green fluorescent protein (GFP).