pLCA.66/2272同源重组质粒 货号:P0383
来源:
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作者:shhebio
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发布时间: 2018-03-14
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321 次浏览
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分享到:
信息
别名
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Plasmid #22733
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启动子
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PGK
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复制子
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ori,F1 ori
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终止子
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bGH poly(A) signal
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质粒分类
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基因文库质粒;其它基因质粒;其它种源质粒
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质粒大小
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6678bp
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原核抗性
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Amp
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真核抗性
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Puro
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克隆菌株
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DH5α
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培养条件
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37℃
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质粒宿主
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哺乳细胞
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质粒用途
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基因编辑
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原核抗性
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氨苄青霉素
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真核抗性
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嘌呤霉素
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简介
This vector was designed for assembling gene targeting constructs using the BAC recombineering approach. The vector contains a puromycin-(delta)thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pUDTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo). These selectable markers are flanked by lox66 and lox2272 sites (Cre-recombinase recognition sequences) in a pBluescript KS+ backbone with a modified multi-cloning region.
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