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pLCA.66/2272同源重组质粒 货号:P0383

信息

别名 Plasmid #22733
启动子 PGK
复制子 ori,F1 ori
终止子 bGH poly(A) signal
质粒分类 基因文库质粒;其它基因质粒;其它种源质粒
质粒大小 6678bp
原核抗性 Amp
真核抗性 Puro
克隆菌株 DH5α
培养条件 37℃
质粒宿主 哺乳细胞
质粒用途 基因编辑
原核抗性 氨苄青霉素
真核抗性 嘌呤霉素


简介

      This vector was designed for assembling gene targeting constructs using the BAC recombineering approach. The vector contains a puromycin-(delta)thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pUDTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo). These selectable markers are flanked by lox66 and lox2272 sites (Cre-recombinase recognition sequences) in a pBluescript KS+ backbone with a modified multi-cloning region.

      

图谱


garmin 230