pGL4.22[luc2CP/Puro]哺乳报告质粒 货号:P2437
来源:
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作者:shhebio
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发布时间: 2018-04-13
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169 次浏览
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信息
复制子
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PUC
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原核抗性
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Amp
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真核抗性
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Puro
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克隆菌株
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DH5α
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培养条件
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37℃
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质粒宿主
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哺乳细胞
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质粒用途
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信号报告
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片段类型
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启动子
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片段物种
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空载体
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原核抗性
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氨苄青霉素
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真核抗性
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嘌呤霉素
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荧光标记
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Fluc
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简介
pGL4.22[luc2CP/Puro]是一个哺乳细胞单荧光素酶报告质粒。The pGL4.22[luc2CP/Puro]
Vector encodes the luciferase reporter gene luc2CP (Photinus pyralis) and is
designed for high expression and reduced anomalous transcription. This vector
contains a mammalian selectable marker for puromycin resistance in which the
number of transcription factor binding sites has been reduced and mammalian
codon usage optimized. The vector is engineered with fewer consensus regulatory
sequences for reduced background and a decreased risk of anomolous
transcription and has a synthetic reporter gene, which has been codon optimized
for mammalian expression. The pGL4.22[luc2CP/Puro] Vector is a basic vector
with no promoter. However, it contains a multiple cloning region that allows
cloning of a promoter of choice. The luc2CP reporter gene contains two protein
destabilization sequences, hCL1 and hPEST. The protein encoded by luc2CP
responds more quickly and with greater magnitude to changes in transcriptional
activity than the luc2 gene, its more stable counterpart.
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