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pPR3-N膜蛋白双杂交质粒 货号:P5092

信息

启动子 CYC1 promoter
复制子 pUC ori,F1 ori,2μ ori
终止子 CYC1 terminator
质粒分类 酵母细胞质粒;酵母杂交质粒;双杂交类质粒
质粒大小 6200bp
质粒标签 N-NubG;N-HA
原核抗性 Amp
真核抗性 TRP1
克隆菌株 DH5α
培养条件 37℃
表达宿主 酵母菌
培养条件 30℃,YPD,有氧
5'测序引物 CYC1-F:CTTTCCTTATACATTAGGACC
3'测序引物 CYC1-R:GGGACCTAGACTTCAGGTTG
备注 高拷贝质粒
质粒宿主 酵母菌
质粒用途 杂交
原核抗性 氨苄青霉素
真核抗性 TRP1


简介

         pPR3-N是一个用于膜蛋白酵母双杂交实验的质粒。The DUALmembrane system is intended for the detection and identification of interactions involving integral membrane proteins and membrane-associated proteins in yeast. The DUALmembrane pairwise interaction kit enables you to:
        Investigate the interaction between a membrane protein (integral or membrane-associated) and a membrane protein or soluble protein
        Map domains or amino acids which are critical for an interaction
        Screen cDNA libraries using a membrane protein as a bait to find novel
        interacting proteins (requires purchase of a separate cDNA library from Dualsystems)



图谱


garmin 230