pECTO金黄色葡萄球菌染色体基因异位互补质粒 货号：P0434
来源:
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作者:shhebio
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发布时间: 2018-04-16
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833 次浏览
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信息
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别名
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金黄葡萄球染体异位互补
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原核抗性
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Amp
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克隆菌株
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DH5α
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培养条件
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37℃
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质粒宿主
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金黄色葡萄球菌
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质粒用途
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蛋白表达
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原核抗性
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氨苄青霉素
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简介
pMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureu.pECTO a Gram negative/Gram positive shuttle vector derived from pMAD2 designed to integrate DNA sequences between SAOUHSC_00278 and SAOUHSC_00279 (NCTC8325 nomenclature HG003). It carries about 1kb of both sequences. A terminator introduced between the SAOUHSC_00278 and SAOUHSC_00279 sequences prevents polar effects associated with the expression of inserted genes.This a Gram negative/Gram positive shuttle vector with two specialized replicons and antibiotic markers. pBR322 replicon and ampicillin resistance for E. coli. pE194(ts) replicon and erythromycin resistance for S. aureus. In S. aureus, the plasmid propagates at T<30°C, it becomes a suicide vector at T>37°C.
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