信息

启动子	SV40，T7，T3
复制子	pBR322
质粒分类	哺乳细胞，蛋白过表达载体
质粒大小	7,9kb
原核抗性	Amp
真核抗性	G418
克隆菌株	DH5α
培养条件	37℃
表达宿主	哺乳细胞
诱导方式	无须诱导，瞬时表达
5'测序引物	T7:TAATACGACTCACTATAGGG
3'测序引物	BGH:TAGAAGGCACAGTCGAGG
质粒宿主	大肠杆菌
质粒用途	基因模板
片段类型	噬菌体
片段物种	空载体
原核抗性	氨苄青霉素

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简介

       SuperCos 1 is a novel, 7.9-kb cosmid vector that contains bacteriophage promoter sequences flanking a unique cloning site (see Figure 1). This structure allows rapid synthesis of "walking" probes specific for the extreme ends of insert DNA. The SuperCos 1 vector is also engineered to contain genes for the amplification and expression of cosmid clones in eukaryotic cells. In addition, most genomic inserts can be excised as a single large restriction fragment using the Not I restriction site that flanks the SuperCos 1 polylinker.

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