pComb3XLambda噬菌体展示质粒 货号：P1024
来源:
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作者:shhebio
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发布时间: 2017-11-28
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170 次浏览
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信息
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别名
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Plasmid #63892
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启动子
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Lac/lac promoter
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复制子
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pBR322F2
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质粒大小
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4772bp
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质粒标签
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C-6×His，C-HA
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原核抗性
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Amp
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克隆菌株
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TG1
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培养条件
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37℃
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质粒宿主
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大肠杆菌
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质粒用途
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基因模板
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片段类型
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噬菌体
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片段物种
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空载体
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原核抗性
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氨苄青霉素
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简介
Backbone pComb3X containing PCR template for human antibody lambda light chain constant region.
pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of an asymmetric SfiI cassette for directional cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XLambda can be used as a template to amplify the lambda light chain constant region for construction of chimeric antibody libraries as described in:
Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York
Please note that there is a A246V mutation in heavy chain region; however, this vector is meant to be used as a PCR template for the light chain region from base# 613-1016.
本质粒参考文献：Methods for the generation of chicken monoclonal antibody fragments by phage display.
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