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网站首页 >> pGEX-6P-2大肠表达质粒 货号：P1041
pGEX-6P-2大肠表达质粒 货号：P1041

信息

别名 pGEX6P2;pGEX6P-2
启动子 Tac
复制子 pBR322
质粒分类 大肠杆菌载体；PGEX系列表达质粒
质粒大小 4985bp
质粒标签 N-GST，N-PP/3C
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 大肠杆菌
培养条件 37℃，有氧，LB
诱导方式 IPTG或乳糖及其类似物
5'测序引物 pGEX5: GGGCTGGCAAGCCACGTTTGGTG
3'测序引物 pGEX3: CCGGGAGCTGCATGTGTCAGAGG
备注 可用GST亲和柱纯化重组蛋白
质粒宿主 大肠杆菌
质粒用途 蛋白表达
片段类型 基因CDS
片段物种 空载体
原核抗性 卡那霉素

简介

There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 µl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 µl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap™ 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty columns with required buffers. For simplified buffer preparation use the GST Buffer Kit. Ordering information for all associated products is listed below.

图谱

garmin 230