pSNAP-Tag(T7)2大肠表达质粒 货号：P1296
来源:
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作者:shhebio
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发布时间: 2017-12-06
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191 次浏览
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分享到:
信息
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启动子
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T7 promoter
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复制子
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Ori，F1 ori
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终止子
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rrnB T1 terminator
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质粒分类
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大肠杆菌质粒；大肠其它质粒；其它大肠质粒
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质粒大小
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6495bp
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质粒标签
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SNAP-tag
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原核抗性
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Amp
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克隆菌株
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DH5α
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培养条件
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37℃
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质粒宿主
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大肠杆菌
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原核抗性
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氨苄青霉素
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简介
pSNAP-Tag(T7)2是一个大肠杆菌表达质粒，T7启动子驱动SNAP标签和目的基因融合表达。pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag® protein. Expression is under control of the IPTG inducible T7 promoter. The pSNAP-tag(T7)-2 Vector can also be used as a control plasmid expressing the SNAP-tag protein (20 kDa). The target gene should be cloned as a fusion to the Nor C-terminus of the SNAP-tag. The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6 - alkylguanine-DNA-alkyltransferase (hAGT)(1,2,3). SNAP-tag substrates are derivatives of benzyl purines and benzyl pyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Cloning and expression of SNAP-tag fusion proteins are described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with SNAPtag substrates.
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