pCDNA3.1-3×HA-N哺乳表达质粒 货号：P2011
来源:
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作者:shhebio
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发布时间: 2017-12-06
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148 次浏览
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分享到:
信息
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启动子
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CMV
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复制子
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pUC
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终止子
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BGH poly（A）signal
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质粒分类
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哺乳系列质粒；哺乳表达质粒；pCDNA系列质粒
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质粒大小
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5541bp
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质粒标签
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N-3×HA
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原核抗性
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Amp
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真核抗性
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G418
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克隆菌株
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DH5α
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培养条件
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37℃
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表达宿主
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293T等哺乳细胞
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培养条件
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37℃，5%CO2
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诱导方式
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无需诱导
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5'测序引物
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pCDNA3.1-F（CTAGAGAACCCACTGCTTAC）
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3'测序引物
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pCDNA3.1-R（TAGAAGGCACAGTCGAGG）
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质粒宿主
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哺乳细胞
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质粒用途
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蛋白表达
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片段类型
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基因CDS
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片段物种
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空载体
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原核抗性
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氨苄青霉素
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简介
pCDNA3.1-3×HA-N质粒是一种高效表达的哺乳质粒。该质粒含有Amp抗性，可转至DH5α中转化。培养条件为LB培养基，37℃。
pCDNA3.1-3×HA-N vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin selectable marker and a forward-orientation multiple cloning site.High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells Neomycin resistance gene for selection of stable cell lines. There are three HA tags on the N side.Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen.
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