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pIRES2-EGFP哺乳荧光质粒 货号:P2085

信息

别名 pIRES-EGFP,pIRES2EGFP
启动子 CMV
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞,荧光蛋白报告载体
质粒大小 5308bp
原核抗性 Kan
真核抗性 G418
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导,瞬时表达
5'测序引物 pIRES2-EGFP.P5:GTAGGCGTGTACGGTGGGAG
3'测序引物 pIRES2-EGFP.P3:AACGCACACCGGCCTTATTC
质粒宿主 哺乳细胞
质粒用途 蛋白表达
片段类型 基因CDS
原核抗性 卡那霉素
荧光标记 绿色


简介
      pIRES2-EGFP contains the internal ribosome entry site (IRES; 1, 2) of the encephalomyocarditis virus (ECMV) between the MCS and the enhanced green fluorescent protein (EGFP) coding region.This permits both the gene of interest (cloned into the MCS) and the EGFP gene to be translated from a single bicistronic mRNA. pIRES2-EGFP is designed for the efficient selection (by flow cytometry or other methods) of transiently transfected mammalian cells expressing EGFP and the protein of interest. This vector can also be used to express EGFP alone or to obtain stably transfected cell lines without time-consuming drug and clonal selection.



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garmin 230