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网站首页 >> pGL3-Enhancer哺乳报告质粒 货号：P2132
pGL3-Enhancer哺乳报告质粒 货号：P2132

信息

别名 pGL3.0-Enhancer
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞，信号通路报告载体
质粒大小 5064bp
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 RVP3:CTAGCAAAATAGGCTGTCCC
3'测序引物 根据序列设计引物
质粒宿主 哺乳细胞
质粒用途 信号报告
片段类型 启动子
原核抗性 氨苄青霉素
荧光标记 Fluc

简介
     The pGL3 Luciferase Reporter Vectors(a–c) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.


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