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网站首页 >> pGL4.19[luc2CP-Neo]哺乳报告质粒 货号：P2138
pGL4.19[luc2CP-Neo]哺乳报告质粒 货号：P2138

信息

启动子 SV40
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞，信号通路报告载体
质粒大小 5778bp
原核抗性 Amp
真核抗性 G418
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 Sv40-polyA-R：GAAATTTGTGATGCTATTGC
3'测序引物 根据序列设计引物
质粒宿主 哺乳细胞
质粒用途 信号报告
片段类型 启动子
片段物种 空载体
原核抗性 氨苄青霉素
荧光标记 Fluc

简介
     The pGL4.19[luc2CP/Neo] Vector encodes the luciferase reporter gene luc2CP (Photinus pyralis) and is designed  for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker  for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian  codon usage optimized. This vector is engineered with fewer consensus regulatory sequences for reduced background  and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized  for mammalian expression. The pGL4.19[luc2CP/Neo] Vector is a basic vector with no promoter. However, it contains  a multiple cloning region that allows cloning of a promoter of choice. The luc2CP reporter gene contains two protein  destabilization sequences, hCL1 and hPEST. The protein encoded by luc2CP responds more quickly and with greater  magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.


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garmin 230