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网站首页 >> pBIFC-bFosVC155双分荧光质粒 货号：P2181
pBIFC-bFosVC155双分荧光质粒 货号：P2181

信息

别名 Plasmid: #22013
启动子 CMV
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞，双分子荧光互补载体
质粒大小 4352bp
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物 根据序列设计引物
质粒宿主 哺乳细胞
质粒用途 信号报告
原核抗性 氨苄青霉素

简介
     Bimolecular fluorescence complementation (BiFC) analysis enables direct visualization of protein interactions in living cells. The BiFC assay is based on the discoveries that two non-fluorescent fragments of a fluorescent protein can form a fluorescent complex and that the association of the fragments can be facilitated when they are fused to two proteins that interact with each other. BiFC must be confirmed by parallel analysis of proteins in which the interaction interface has been mutated. The BiFC assay has been used for the visualization of interactions between many types of proteins in different subcellular locations and in different cell types and organisms. It is technically straightforward and can be performed using a regular fluorescence microscope and standard molecular biology and cell culture reagen


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