pFUSE-hIgG1e1-Fc2哺乳抗体质粒 货号：P2353
来源:
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作者:shhebio
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发布时间: 2017-12-30
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208 次浏览
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分享到:
信息
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启动子
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EF1α
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复制子
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pUC
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终止子
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SV40 poly(A) signal
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质粒分类
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哺乳细胞，抗体表达载体
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质粒大小
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4194bp
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原核抗性
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Zeo
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真核抗性
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Zeo
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克隆菌株
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DH5α
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培养条件
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37℃
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表达宿主
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哺乳细胞
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诱导方式
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无须诱导，瞬时表达
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5'测序引物
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根据序列设计引物
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3'测序引物
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根据序列设计引物
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质粒宿主
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哺乳细胞
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质粒用途
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蛋白表达
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原核抗性
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博来霉素
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真核抗性
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博来霉素
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简介
pFUSE-Fc is a family of plasmid developed to facilitate the construction of Fc-fusion proteins by fusing the effector region of a protein to the Fc region of an immunoglobul in G (IgG).
pFUSE-Fc plasmids yield high levels of Fc-fusion proteins. The level of expression is usually in the μg/mL range. They can be transfected in a variety of mammalian cells, including myeloma cell lines, CHO cells, monkey COS cells and human embryonic kidney (HEK)293 cells, cells that are commonly used in protein purification systems.
pFUSE-Fc2 (IL2ss) plasmids allow the secretion of Fc-Fusion proteins.They contain the IL2 signal sequence (IL2ss) for the generation of Fc- Fusion proteins derived from proteins that are not naturally secreted. As Fc- Fusion proteins are secreted, they can be easily detected in the supernatant of pFUSE-Fc-transfected cells by SDS-PAGE. Furthermore, functional domains can be identified by immunoblotting and ligand blotting.Fc-Fusion proteins can be easily purified by single-step protein A or protein G affinity chromatography.
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