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pCEP4-CAT哺乳表达质粒 货号:P2363

信息

别名 pCEP4/CAT
启动子 CMV
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞,蛋白过表达载体
质粒大小 10891bp
原核抗性 Amp
真核抗性 Hyg
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导,瞬时表达
5'测序引物 CMV-F:CGCAAATGGGCGGTAGGCGTG
3'测序引物 Sv40-polyA-R:GAAATTTGTGATGCTATTGC
质粒宿主 哺乳细胞
质粒用途 蛋白表达
片段类型 基因CDS
片段物种 空载体
原核抗性 氨苄青霉素
真核抗性 潮霉素


简介
      pCEP4 is an episomal mammalian expression vector that uses thecytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells.
       pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.
         pCEP4是pCEP4/CAT的对照质粒,插入一个基因CAT.



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