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网站首页 >> pLysS质粒 货号：P223
pLysS质粒 货号：P223

信息

启动子 T7Phi3.8
复制子 p15
质粒分类 有毒蛋白表达配套质粒
质粒大小 4886bp
质粒标签 T7 lysozyme
原核抗性 Chl
克隆菌株 DH5α
培养条件 37℃
表达宿主 大肠杆菌
培养条件 参考相关文献
5'测序引物 根据序列设计
3'测序引物 根据序列设计
备注 低拷贝
质粒宿主 广宿主
原核抗性 氯霉素

简介
         pLysS and pLysE are 4886bp plasmids constructed by insertion of the T7 lysozyme gene into the BamH I site of pACYC184 (1, 2). These plasmids are not cloning vectors; they are used in λDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase. The two plasmids differ only by the orientation of the T7 lysozyme gene. In pLysS the T7 lysozyme coding sequence is in the antisense orientation relative to the tet promoter, so only a small amount of T7 lysozyme is produced. In pLysE large amounts of T7 lysozyme are produced from the tet promoter. The construct also contains the weak T7 φ3.8 promoter immediately following the lysozyme gene. The p15A origin of replication is compatible with those found in pBR322- and pUC-derived plasmids.Unique sites are shown on the circle map.
        1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.
        2. Chang, A.C.Y. and Cohen, S.N. (1978) J. Bacteriol. 134, 1141.



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garmin 230