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网站首页 >> pGFPuv大肠荧光质粒 货号：P1315
pGFPuv大肠荧光质粒 货号：P1315

信息

复制子 PUC
原核抗性 Amp
真核抗性 绿色荧光蛋白GFPuv
克隆菌株 DH5α
培养条件 37℃
质粒宿主 大肠杆菌
质粒用途 蛋白表达
片段类型 基因CDS
原核抗性 氨苄青霉素
荧光标记 绿色

简介

           pGFPuv是一个大肠杆菌绿色荧光表达质粒。pGFPuv carries the“cycle 3”variant of GFP described by Crameri et al. (1). This gene was cloned between the two MCSs of the pUC19 derivative pPD16.43 (2). The GFPuv gene canbe easily excised from pGFPuv. Alternatively, the GFPuv coding sequence can be amplified by PCR. The GFPuv gene was inserted in frame with the lacZ initiation codon from pUC19 so that a β-galactosidase-GFPuv fusionprotein is expressed from the lac promoter in E. coli . Note, however, that if you excise the GFPuv coding sequence using a restriction site in the 5' MCS, the resulting fragment will encode the native (i.e.,non-fusion) GFPuv protein. The pUC backbone of pGFPuv provides a high copy number origin of replication and ampicillin resistance gene for propagation in E. coli.

图谱


garmin 230