pMD18-Control大肠克隆对照质粒 货号：P1009
来源:
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作者:shhebio
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发布时间: 2017-11-28
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291 次浏览
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基本信息
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启动子
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Lac/lac promoter
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复制子
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pBR322
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质粒大小
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2692bp
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质粒标签
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LacZ
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原核抗性
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Amp
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克隆菌株
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DH5α
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培养条件
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37℃
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5'测序引物
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M13R:CAGGAAACAGCTATGACC
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3'测序引物
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M13F:TGTAAAACGACGGCCAGT
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质粒宿主
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大肠杆菌
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质粒用途
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基因模板
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片段类型
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基因CDS
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片段物种
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空载体
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原核抗性
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氨苄青霉素
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质粒简介
pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for this Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425
质粒图谱：
