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pACYC177大肠克隆质粒 货号：P1011
来源:
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作者:shhebio
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发布时间: 2017-11-28
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174 次浏览
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分享到:
基本信息
| 复制子 | p15A |
| 质粒大小 | 3941bp |
| 原核抗性 | Amp/Kan |
| 克隆菌株 | DH5α |
| 培养条件 | 37℃ |
| 5'测序引物 | 根据序列设计 |
| 3'测序引物 | 根据序列设计 |
| 备注 | 丰富的酶切位点 |
| 质粒宿主 | 大肠杆菌 |
| 质粒用途 | 基因模板 |
| 片段类型 | 基因CDS |
| 片段物种 | 空载体 |
质粒简介
pACYC177 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC177 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be amplified with chloramphenicol or spectinomycin.
Enzymes with unique restriction sites are shown in bold type and enzymes with two restriction sites are shown in regular type. Location of sites of all NEB restriction enzymes can be found on the NEB web site (choose Technical Reference > DNA Sequences and Maps). Restriction site coordinates refer to the position of the 5′-most base on the top strand in each recognition sequence.
Enzymes with unique restriction sites are shown in bold type and enzymes with two restriction sites are shown in regular type. Location of sites of all NEB restriction enzymes can be found on the NEB web site (choose Technical Reference > DNA Sequences and Maps). Restriction site coordinates refer to the position of the 5′-most base on the top strand in each recognition sequence.
质粒图谱
质粒序列
LOCUS Exported 3941 bp ds-DNA circular SYN 26-8-2015
DEFINITION synthetic circular DNA
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3941)
TITLE Direct Submission
http://www.shhebio.com
FEATURES Location/Qualifiers
source 1..3941
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 783..1328
/direction=RIGHT
/note="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
CDS 1923..2738
/codon_start=1
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
CDS 1923..2738
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
promoter 3594..3698
/gene="bla"
/note="AmpR promoter"
CDS 3699..618
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
61 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
301 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct
781 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
841 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
901 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
961 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
1021 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
1081 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
1141 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
1201 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
1261 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
1321 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
1381 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
1441 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
1501 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
1561 ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg
1621 ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac
1681 ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca
1741 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
1801 gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
1861 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
1921 ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg
1981 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
2041 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
2101 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
2161 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
2221 cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
2281 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
2341 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
2401 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
2461 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
2521 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
2581 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
2641 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
2701 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
2761 aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatcgaac
2821 ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc
2881 aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg
2941 ctccctcact ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc
3001 ttcttcacga ggcagacctc agcgctcaaa gatgcagggg taaaagctaa ccgcatcttt
3061 accgacaagg catccggcag ttcaacagat cgggaagggc tggatttgct gaggatgaag
3121 gtggaggaag gtgatgtcat tctggtgaag aagctcgacc gtcttggccg cgacaccgcc
3181 gacatgatcc aactgataaa agagtttgat gctcagggtg tagcggttcg gtttattgac
3241 gacgggatca gtaccgacgg tgatatgggg caaatggtgg tcaccatcct gtcggctgtg
3301 gcacaggctg aacgccggag gatcctagag cgcacgaatg agggccgaca ggaagcaaag
3361 ctgaaaggaa tcaaatttgg ccgcaggcgt accgtggaca ggaacgtcgt gctgacgctt
3421 catcagaagg gcactggtgc aacggaaatt gctcatcagc tcagtattgc ccgctccacg
3481 gtttataaaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
3541 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
3601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
3661 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
3721 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
3781 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
3841 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
3901 gcacttttaa agttctgcta tgtggcgcgg tattatcccg t
//
DEFINITION synthetic circular DNA
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3941)
TITLE Direct Submission
http://www.shhebio.com
FEATURES Location/Qualifiers
source 1..3941
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 783..1328
/direction=RIGHT
/note="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
CDS 1923..2738
/codon_start=1
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
CDS 1923..2738
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
promoter 3594..3698
/gene="bla"
/note="AmpR promoter"
CDS 3699..618
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
61 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
301 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct
781 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
841 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
901 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
961 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
1021 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
1081 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
1141 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
1201 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
1261 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
1321 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
1381 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
1441 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
1501 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
1561 ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg
1621 ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac
1681 ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca
1741 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
1801 gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
1861 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
1921 ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg
1981 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
2041 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
2101 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
2161 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
2221 cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
2281 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
2341 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
2401 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
2461 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
2521 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
2581 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
2641 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
2701 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
2761 aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatcgaac
2821 ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc
2881 aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg
2941 ctccctcact ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc
3001 ttcttcacga ggcagacctc agcgctcaaa gatgcagggg taaaagctaa ccgcatcttt
3061 accgacaagg catccggcag ttcaacagat cgggaagggc tggatttgct gaggatgaag
3121 gtggaggaag gtgatgtcat tctggtgaag aagctcgacc gtcttggccg cgacaccgcc
3181 gacatgatcc aactgataaa agagtttgat gctcagggtg tagcggttcg gtttattgac
3241 gacgggatca gtaccgacgg tgatatgggg caaatggtgg tcaccatcct gtcggctgtg
3301 gcacaggctg aacgccggag gatcctagag cgcacgaatg agggccgaca ggaagcaaag
3361 ctgaaaggaa tcaaatttgg ccgcaggcgt accgtggaca ggaacgtcgt gctgacgctt
3421 catcagaagg gcactggtgc aacggaaatt gctcatcagc tcagtattgc ccgctccacg
3481 gtttataaaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
3541 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
3601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
3661 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
3721 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
3781 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
3841 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
3901 gcacttttaa agttctgcta tgtggcgcgg tattatcccg t
//
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标题摘要内容