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网站首页 >> pETBlue-2大肠表达质粒 货号：P1128
pETBlue-2大肠表达质粒 货号：P1128

信息

别名 pETBlue2
启动子 T7
复制子 pBR322
终止子 T7 terminator
质粒分类 大肠杆菌载体；PET系列表达质粒
质粒大小 3653bp
质粒标签 C-HSV，C-6×His
原核抗性 Amp
真核抗性 LacZ
克隆菌株 DH5α
培养条件 37℃
表达宿主 大肠杆菌BL21(DE3)pLacI
培养条件 37℃，有氧，LB
诱导方式 IPTG或乳糖及其类似物
5'测序引物 T7:TAATACGACTCACTATAGGG
3'测序引物 T7-ter:TGCTAGTTATTGCTCAGCGG
质粒宿主 大肠杆菌
质粒用途 蛋白表达
片段类型 基因CDS
片段物种 空载体
原核抗性 氨苄青霉素

简介
       The pETBlue™-2 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-2 defines the open reading frame and inserts must be cloned in-frame if expression is desired. The vector features an expanded multiple cloning site (MCS) and optional C-terminal HSV•Tag® and His•Tag® sequences. The sequence is numbered from the first base of the T7 promoter sequence.



图谱

garmin 230