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网站首页 >> pMal-c5X大肠表达质粒 货号：P1153
pMal-c5X大肠表达质粒 货号：P1153

信息

别名 pMalc5X
启动子 Tac
复制子 pBR322
终止子 rrnB T1 terminator
质粒分类 大肠杆菌载体；pMal系列表达质粒
质粒大小 5677bp
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 大肠杆菌BL21(DE3)
培养条件 37℃，有氧，LB
诱导方式 IPTG或乳糖及其类似物
质粒宿主 大肠杆菌
质粒用途 蛋白表达
片段类型 基因CDS
片段物种 空载体
原核抗性 氨苄青霉素

简介
      The vector pMAL-c5X is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Factor Xa .
     MBP fusions made with this vector are expressed cytoplasmically. The MBP has been engineered for tighter binding to amylose resin. A gene or open reading frame is inserted into a restriction site of the vector polylinker, in the same translational reading frame as the malE gene (encoding maltose-binding protein). The fusion protein thus produced can be purified by amylose affinity chromatography. The sequence coding for the four amino acids Ile-Glu-Gly-Arg is present just upstream of the XmnI site. This allows the protein of interest to be cleaved from maltose-binding protein with the specific protease Factor Xa. Fragments inserted in the XmnI site (cleaves GAAGG↓ATTTC) will produce a fusion protein that, after Factor Xa cleavage, contains no vector-derived residues on the protein of interest.


图谱



garmin 230