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网站首页 >> pTrcHis2B大肠表达质粒 货号:P1198
pTrcHis2B大肠表达质粒 货号:P1198

信息

启动子: Trc/lac
复制子: PBR322 ori
终止子: rrnB TT
质粒分类: 大肠杆菌载体；pTrc系列表达质粒
质粒大小: 4404bp
质粒标签: C-His，C-Myc
原核抗性: 氨苄青霉素Amp
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: 大肠杆菌
培养条件: 37℃，有氧，LB
诱导方式: IPTG或乳糖及其类似物
5'测序引物: pTrcHis-F: GAGGTATATATTAATGTATCG
3'测序引物: pTrcHis-R:GATTTAATCTGTATCAGG
备注: pTrc表达质粒

简介

The pTrcHis2 plasmids are pBR322-derived expression vectors designed for efficient recombinant protein expression and purification in E. coli. High levels of expression are possible using the trc (trp-lac) promoter (Egon et al., 1983) and the rrnB anti-termination region (Li et al., 1984). The trc promoter contains the –35 region of the trp promoter together with the –10 region of the lac promoter (Brosius et al., 1985; Egon et al., 1983; Mulligan et al., 1985). To regulate expression, the gene encoding Lac repressor (lacIq) is provided in the pTrcHis2 vectors, allowing regulation of the trc promoter regardless of whether the host strain contains a gene encoding the Lac repressor.
    Isopropyl-Beta-D-thiogalactopyranoside (IPTG) is used to induce expression of your gene. Translation is enhanced by the bacteriophage T7 gene 10 translation enhancer and a minicistron that provides highly efficient translational restart into the open reading frame of the multiple cloning site. DNA inserts are positioned downstream and in frame with the initiation ATG and a C-terminal fusion peptide. The C-terminal peptide encodes the myc epitope and six histidine residues that function as a metal binding site in the expressed protein.

图谱

garmin 230