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网站首页 >> pKK223-3大肠表达质粒 货号：P1206
pKK223-3大肠表达质粒 货号：P1206

信息

启动子 Tac
复制子 pBR322
终止子 rrnB T1
质粒分类 大肠杆菌载体；蛋白表达质粒
质粒大小 4584bp
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 大肠杆菌
培养条件 37℃，有氧，LB
诱导方式 IPTG或乳糖及其类似物
备注 表达质粒
质粒宿主 大肠杆菌
质粒用途 蛋白表达
片段类型 基因CDS
片段物种 空载体
原核抗性 氨苄青霉素

简介
pKK223-3 (1) contains the strong tac promoter upstream from the multiple cloning site and the strong rrnB ribosomal terminator downstream for control of protein expression (2-4). Induction: tac promoter is inducible with 1-5 mM IPTG. Promoter is very strong; even uninduced cells may show a low level of expression. Expression: Genes containing a ribosome-binding site and ATG can be inserted into any unique site in the MCS for expression. The ribosomebinding site on the plasmid can be utilised to express inserts if the ATG start codon of the insert is within 5-13 bp from the provided ribosomebindingsite (5, 6). Transcription terminators: rrnB transcription terminators stabilize the plasmid by inhibiting read-through transcription initiated from the tac promoter in the parent plasmid. Host(s): lac Iq strains; E. coli JM105 is recommended. Selectable marker(s): Plasmid confers resistance to 100 μg/ml ampicillin. Amplification: Recommended. Control Regions: Expression control region: tac promoter: -10: 50-44; -35: 72-67; Ribosome binding site: 11. MCS: 4552-4587. rrnB operon region: 5S rRNA region: 4471-4352; rrnB T1 terminator: 4348-4305; rrnB T2 terminator: 4173-4146. b-lactamase gene region: Promoter: -10: 4082-4077; -35: 4105-4100; Start codon (ATG): 4035; Stop codon (TAA): 3178. Plasmid replication region: Site of replication initiation: 2417-2415; Region necessary for replication: 3111-2415


 

图谱




garmin 230