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pTYB1大肠表达质粒 货号:P1287

信息

启动子 T7
复制子 M13
质粒分类 大肠杆菌载体;蛋白表达质粒
质粒大小 7477bp
质粒标签 Intein
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 大肠杆菌
培养条件 37℃,有氧,LB
5'测序引物 T7:TAATACGACTCACTATAGGG
3'测序引物 根据序列设计
备注 表达质粒
质粒宿主 大肠杆菌
质粒用途 蛋白表达
片段类型 基因CDS
片段物种 空载体
原核抗性 氨苄青霉素


简介
     The pTYB1 is an E. coli vector created for fusion protein expression and purification. A target gene can be inserted into the the ORF by use of the MCS upstream of the Sce VMA intein. The NdeI site in the MCS has the initiator sequence ATG that enables translation of the ORF. The chitin-binding domain(CBD) downstream of the intein results in a target protein-intein with C-terminal chitin beads that facilitate protein purification. Release of the protein is induced by the thiol cleavage activity of the intein.



图谱


garmin 230