该质粒可用于在鸡b-肌动蛋白，兔b-球蛋白杂合启动子（CAG）的控制下高效表达基因，和各种哺乳动物细胞中的人CMV-IE增强子; CAG启动子序列由鸡b-肌动蛋白启动子，第一外显子和第一内含子的一部分（似乎具有强的增强子样活性），与兔b-珠蛋白片段连接，由第二内含子的3'部分 包括正常拼接反应所需的分支点）和第三外显子的5'部分。
This plasmid is useful for highly efficient expression of genes under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.The AG promoter sequence consists of the chicken β-actin promoter, the first exon and part of the first intron (that seems to have a strong enhancer-like activity) linked to a rabbit β-globin fragment, consisting of a 3' part of the second intron (inclusive a branch point which is required for normal splicing reactions) and a 5' part of the third exon.When cloning a fragment downstream from the lac promoter it may be advisable to use lacIq strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
