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网站首页 >> pGL3-Basic哺乳报告质粒 货号：P2129
pGL3-Basic哺乳报告质粒 货号：P2129

信息

别名 pGL3.0-Basic
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞，信号通路报告载体
质粒大小 4818bp
原核抗性 Amp
真核抗性 荧光素酶Luc
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 RVP3:CTAGCAAAATAGGCTGTCCC
3'测序引物 GLP2:CTTTATGTTTTTGGCGTCTTCC
质粒宿主 哺乳细胞
质粒用途 信号报告
片段类型 启动子
片段物种 空载体
原核抗性 氨苄青霉素
荧光标记 Fluc

简介
    The pGL3 Luciferase Reporter Vectors(a–c) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.


图谱




garmin 230