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网站首页 >> pRL-CMV哺乳报告质粒 货号：P2150
pRL-CMV哺乳报告质粒 货号：P2150

信息

启动子 CMV
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞，信号通路报告载体
质粒大小 4079bp
原核抗性 Amp
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物 Sv40-polyA-R：GAAATTTGTGATGCTATTGC
质粒宿主 哺乳细胞
质粒用途 信号报告
原核抗性 氨苄青霉素
荧光标记 Fluc

简介
    The pRL-CMV Vector may be used in combination with any experimental reporter vector to co-transfect mammalian cells. However, it is important to realize that trans effects between promoters on co-transfected plasmids can potentially affect reporter gene expression . Primarily, this is of concern when either the control or experimental reporter vector, or both, contain very strong promoter/enhancer elements (such as CMV). The occurrence and magnitude of such effects will depend on several factors: a) the combination and activities of the genetic regulatory elements present on the co-transfected vectors, b) the relative ratio of experimental vector to control vector introduced into the cells, and c) the cell type transfected.


图谱



garmin 230