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网站首页 >> pMIR-Report Luciferase哺乳报告质粒 货号：P2155
pMIR-Report Luciferase哺乳报告质粒 货号：P2155

信息

启动子 CMV
复制子 pUC
终止子 SV40 poly(A) signal
质粒分类 哺乳细胞，信号通路报告载体
质粒大小 6470bp
原核抗性 Amp
真核抗性 Puro
克隆菌株 DH5α
培养条件 37℃
表达宿主 哺乳细胞
诱导方式 无须诱导，瞬时表达
5'测序引物 CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物 M13F：TGTAAAACGACGGCCAGT
质粒宿主 哺乳细胞
质粒用途 信号报告
原核抗性 氨苄青霉素
真核抗性 嘌呤霉素

简介
     The pMIR-REPORT™ miRNA Expression Reporter Vector System consists of an experimental firefly luciferase reporter vector and an associated β-gal reporter control plasmid. By inserting predicted miRNA target sequences in the multiple cloning site, pMIR-REPORT Luciferase miRNA Expression Reporter Vector can be used to conduct accurate, quantitative evaluations of miRNA function. It can also be used to evaluate siRNA target sites and to analyze the influence of 3' UTR sequences on gene expression. pMIR-REPORT Luciferase contains a firefly luciferase reporter gene under the control of a CMV promoter/termination system. The 3' UTR of the luciferase gene contains a multiple cloning site for insertion of predicted miRNA binding targets or other nucleotide sequences. By cloning a predicted miRNA target sequence into pMIR-REPORT, the luciferase reporter is subjected to regulation that mimics the miRNA target.


图谱




garmin 230